The Effect of Different Doses of Dexamethasone on The Hepatic Tissue of Pregnant Swiss Albino Mice Mus Masculus

Authors

DOI:

https://doi.org/10.48112/acmr.v5i1.67

Keywords:

Dexamethasone, Liver, pregnant Albino Mice Embryos

Abstract

Abstract Views: 29

The aim of the study is to see how Dexamethasone affects the liver of pregnant Swiss albino mice at various stages of development. The study included Fifty-four pregnant female mice that divided into three cohorts at random. There are 18 pregnant mice in each group. Each member was injected with different doses of Dexamethasone into a specific tail vein at different times. The control group, on the other hand, received standard saline injections. The mice were then killed at days 13, 15 and 17.  And the results illustrated that when different dosages of Dexamethasone were used at different periods, the results demonstrated a negative effect on the liver of pregnant mice. These effects were stronger as the number and concentration of medication doses increased. Dexamethasone produces morphological abnormalities in the liver such as necrosis, vascular congestion, binucleated cells, and microabscesses involving hepatocytes and inflammatory cells. In conclusion, the use of Dexamethasone shows different changes in the liver tissue, including necrosis, Binucleated cells, vascular congestion and micro abscess when it was used for a long-term exposure or by repeating doses.

References

Kajihara H, Malliwah JA, Matsumura M, Taguchi K, Iijima S. Changes in blood cortisol and aldosterone levels and ultrastructure of the adrenal cortex during hemorrhagic shock. Pathol Res Pract. 1983;176(2-4):324-40. doi: 10.1016/S0344-0338(83)80022-3.

Bin Rubaia'an MA, Alotaibi MK, Alotaibi NM, Alqhtani NR. Cortisol in Oral and Maxillofacial Surgery: A Double-Edged Sword. Int J Dent. 2021;2021:7642875. doi: 10.1155/2021/7642875.

Arnaldi G, Scandali VM, Trementino L, Cardinaletti M, Appolloni G, Boscaro M. Pathophysiology of dyslipidemia in Cushing's syndrome. Neuroendocrinology. 2010;92 Suppl 1:86-90. doi: 10.1159/000314213.

Nemmar A, Beegam S, Yuvaraju P, Zaaba NE, Elzaki O, Yasin J, et al. Pathophysiologic effects of waterpipe (shisha) smoke inhalation on liver morphology and function in mice. Life Sci. 2024;336:122058. doi: 10.1016/j.lfs.2023.122058.

Pierce L. Anatomy and physiology of the liver in relation to clinical assessment. Nurs Clin North Am. 1977;12(2):259-73.

Rappaport AM, Borowy ZJ, Lougheed WM, Lotto WN. Subdivision of hexagonal liver lobules into a structural and functional unit; role in hepatic physiology and pathology. Anat Rec. 1954;119(1):11-33. doi: 10.1002/ar.1091190103.

Ruz-Maldonado I, Gonzalez JT, Zhang H, Sun J, Bort A, Kabir I, et al. Heterogeneity of hepatocyte dynamics restores liver architecture after chemical, physical or viral damage. Nat Commun. 2024;15(1):1247. doi: 10.1038/s41467-024-45439-0.

Brown GT, Kleiner DE. Histopathology of nonalcoholic fatty liver disease and nonalcoholic steatohepatitis. Metabolism. 2016;65(8):1080-6. doi: 10.1016/j.metabol.2015.11.008.

Bain S. Pathology of the Fischer Rat — Reference and Atlas. Pathology. 1991;23(4):368. doi: 10.1016/S0031-3025(16)36070-6.

Hunter MP, Wilson CM, Jiang X, Cong R, Vasavada H, Kaestner KH, et al. The homeobox gene Hhex is essential for proper hepatoblast differentiation and bile duct morphogenesis. Dev Biol. 2007;308(2):355-67. doi: 10.1016/j.ydbio.2007.05.028.

Baratta JL, Ngo A, Lopez B, Kasabwalla N, Longmuir KJ, Robertson RT. Cellular organization of normal mouse liver: a histological, quantitative immunocytochemical, and fine structural analysis. Histochem Cell Biol. 2009;131(6):713-26. doi: 10.1007/s00418-009-0577-1.

Lawrence J, Nho R. The Role of the Mammalian Target of Rapamycin (mTOR) in Pulmonary Fibrosis. Int J Mol Sci. 2018;19(3):778. doi: 10.3390/ijms19030778.

Van Merris V, Van Wemmel K, Cortvrindt R. In vitro effects of dexamethasone on mouse ovarian function and pre-implantation embryo development. Reprod Toxicol. 2007;23(1):32-41. doi: 10.1016/j.reprotox.2006.08.008.

Schmidt IG. Central nervous system effects of ethambutol in monkeys. Ann N Y Acad Sci. 1966;135(2):759-74. doi: 10.1111/j.1749-6632.1966.tb45521.x.

Nosel PG, Klein NW. Methionine decreases the embryotoxicity of sodium valproate in the rat: in vivo and in vitro observations. Teratology. 1992;46(5):499-507. doi: 10.1002/tera.1420460514.

Usami M, Tabata H, Ohno Y. Effects of methionine on selenium embryotoxicity in cultured rat embryos. Teratog Carcinog Mutagen. 2002;22(4):301-8. doi: 10.1002/tcm.10025.

New DA. Whole embryo culture, teratogenesis, and the estimation of teratologic risk. Teratology. 1990;42(6):635-42. doi: 10.1002/tera.1420420608.

Garfield SA, Mohamed SA, Cardell RR Jr. The effects of insulin replacement and withdrawal on hepatic ultrastructure and biochemistry. Am J Anat. 1984;170(2):127-42. doi: 10.1002/aja.1001700202.

Striffler J, Cardell EL, Cardell RR Jr. Effects of glucagon on hepatic glycogen and smooth endoplasmic reticulum. Am J Anat. 1981;160(4):363-79. doi: 10.1002/aja.1001600402.

Cardell RR Jr. Action of metabolic hormones on the fine structure of rat liver cells. III. Effects of adrenalectomy and administration of cortisone. Anat Rec. 1974;180(2):309-29. doi: 10.1002/ar.1091800206.

Van Merris V, Van Wemmel K, Cortvrindt R. In vitro effects of dexamethasone on mouse ovarian function and pre-implantation embryo development. Reprod Toxicol. 2007;23(1):32-41. doi: 10.1016/j.reprotox.2006.08.008.

Shi K, Jiang J, Ma T, Xie J, Duan L, Chen R, et al. Dexamethasone attenuates bleomycin-induced lung fibrosis in mice through TGF-β, Smad3 and JAK-STAT pathway. Int J Clin Exp Med. 2014;7(9):2645-50.

Sapolsky RM, Romero LM, Munck AU. How do glucocorticoids influence stress responses? Integrating permissive, suppressive, stimulatory, and preparative actions. Endocr Rev. 2000;21(1):55-89. doi: 10.1210/edrv.21.1.0389.

Lee KH, Chen YS, Judson JP, Chakravarthi S, Sim YM, Er HM. The effect of water extracts of Euphorbia hirta on cartilage degeneration in arthritic rats. Malays J Pathol. 2008;30(2):95-102.

Garfield SA, Scott AC, Cardell RR Jr. Alterations in hepatic fine structure after chronic exposure of rats to dexamethasone. Anat Rec. 1978;192(1):73-87. doi: 10.1002/ar.1091920107.

Yüce A, Ateşşahin A, Ceribaşi AO, Aksakal M. Ellagic acid prevents cisplatin-induced oxidative stress in liver and heart tissue of rats. Basic Clin Pharmacol Toxicol. 2007;101(5):345-9. doi: 10.1111/j.1742-7843.2007.00129.x.

Galliani I, Santi P, Falcieri E. Morphological aspects of steroid hormone action on rat hepatocyte. Boll Soc Ital Biol Sper. 1993;69(3):145-52.

Rogers WA, Ruebner BH. A retrospective study of probable glucocorticoid-induced hepatopathy in dogs. J Am Vet Med Assoc. 1977;170(6):603-6.

Kim M, Shin HK. The water-soluble extract of chicory influences serum and liver lipid concentrations, cecal short-chain fatty acid concentrations and fecal lipid excretion in rats. J Nutr. 1998;128(10):1731-6. doi: 10.1093/jn/128.10.1731.

Published

2024-04-28

How to Cite

Taher, S. G. (2024). The Effect of Different Doses of Dexamethasone on The Hepatic Tissue of Pregnant Swiss Albino Mice Mus Masculus. Advances in Clinical Medical Research, 5(1). https://doi.org/10.48112/acmr.v5i1.67